Objective:
Compare colony forming units (CFUs) on 4 days, 2 different antimicrobial connectors (Baxter V-Link™ Vital Shield™, and RyMed -7001 Nano®) and one zero connector (RyMed-5001 InVision-Plus® with Neutral Advantage™ Technology) in vitro.
Methods:
Independent laboratory tested 2 antimicrobial connectors, one zero reflux connector, 20 connectors each, 3 controls, each of 4 days, same laboratory conditions. Each connector swabbed, inoculated minimum viable 105 each organism (Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli). Appropriate equipment, reagents, media, safety used.
Results: Because RYM-5001® and RYM-7001® produced no CFU for any bacteria consistently across all days for all 4 bacteria types, statistical analysis could not be performed, due to no variability in the estimate on each day for these devices. We plotted mean number of CFUs per device across 4 measurement days for each bacterium. Examining the plots, the Baxter V-Link™ produced 2.5 to 200 times more bacteria over time than the RYM-5001® and RYM-7001®, regardless of bacteria type.
Conclusions: Connector with most and consistent bacteria over 4 days was V-Link™. No consistent bacteria with the RyMed-7001®. The RyMed-5001® non-antimicrobial connector had much less CFUs all 4 bacteria types compared to the antimicrobial V-Link™. Researchers must include technological design as another significant factor in preventing and controlling CRBSIs.