Objective: To develop a Variable Number Tandem Repeat scheme to discriminate between clinical isolates of Klebsiella pneumonia and to compare it with the standard pulsed-field gel electrophoresis (PFGE) method.
Methods: The genome sequence of K.pneumoniae MGH 78578 was put through a tandem repeat finder programme which identified 117 loci with tandemly repeated sequences. Eleven were selected and primers designed from the flanking sequences such that all shared a similar melting temperature. A panel of 24 isolates, representing 20 PFGE defined strains was selected for the study. These isolates came from hospitals in the
Results: Of the eleven VNTR loci screened, seven provided discrimination between isolates. All PFGE defined strains could be distinguished from one another on the basis of their VNTR profiles. Isolates within a PFGE cluster shared the same VNTR profile. The virulent K1 clone of clonal complex (CC) 23, associated with pyogenic liver abscesses, could be unambiguously identified from its characteristic VNTR profile.
Conclusions: A scheme using a combination of seven VNTR loci was as effective as conventional PFGE in distinguishing isolates and provided results more rapidly. Screening for more loci could improve the discriminatory power of the VNTR typing scheme.