Objective: Determine the ability of a mobile ultraviolet light (UV) light unit to reduce bacterial contamination of environmental surfaces in patient rooms.
Methods: An automated mobile UV light unit that emits UV-C (254 nm range) light was placed in patient rooms after patient discharge and terminal cleaning had been performed. UV intensity was set at a target of 22,000 microwatts/cm2 to eradicate spores. D/E neutralizing agar rodac plates were used to obtain cultures of 5 standardized high-touch surfaces in each room before and after UV light decontamination (UVLD). Aerobic colony counts (ACC) were calculated for each surface. To determine log reductions achieved, a modification of ASTM E-2197 quantitative disk carrier test method was used. Stainless steel disk carriers were inoculated with ~ 105 CFUs of Clostridium difficile spores, air-dried, placed in sterile petri dishes and then placed in shadowed and non-shadowed areas of the patient room and bathroom. Three control disks (not exposed to UV light) and 5 disks from each decontaminated room were placed in phosphate buffered saline with 0.1% Tween 80 and vortexed to elute the spores. The eluates were serially diluted and the entire contents were filtered and cultured to determine log reductions achieved. In-room ozone concentrations during UVLD were measured by using a portable ozone monitor (lower limit of detection, 5 PPB).
Results: Preliminary findings are based on UVLD of 5 patient rooms, with cycle times of 40-50 min. Results of ACC are shown below.
| Median ACC (Range) Before UV Decontamination | Median ACC (Range) After UV Decontamination | |
Bedside rail | 10 CFUs (0-53) | 0 CFUs (0) | |
Overbed table | 3 CFUs (0-26) | 0 CFUs (0) | |
Television remote | 18 CFUs (1-104) | 0 CFUs (0-1) | |
Bathroom grab bar | 164 CFUs (9-300) | 44 CFUs (0-160) | |
Toilet seat | 11 CFUs (0-300) | 0 CFUs (0-17) |
12 (92%) of 13 contaminated high-touch surfaces in patient rooms yielded no growth, and 1 surface yielded 1 colony after UVLD. 2 surfaces were culture-negative before and after UVLD. 3 (38%) of 8 contaminated high-touch surfaces in patient bathrooms yielded no growth after UVLD. 4 of the 5 surfaces in bathrooms that yielded growth after UVLD were rough-surfaced grab bars located in shadowed areas. Log10 reductions of C. difficile spores on carrier disks ranged from 0.4 to 2.3. In-room ozone concentrations during UVLD ranged from undetectable to 0.008 PPM.
Conclusions: Our preliminary findings revealed that the mobile UV-C light unit eliminated aerobic bacteria from most of the high-touch surfaces sampled, except bathroom grab bars located in shadowed areas. Further evaluation of this technology is warranted to establish its role in terminal decontamination of patient rooms.