Objective: The aim of this study was to determine the efficacy of a newly formulated mucoadhesive PI antiseptic using a porcine mucosal model designed to simulate mucocilliary clearance in the nares.
Methods: Ex-vivo porcine vaginal mucosal tissue was treated with a 3MTM Skin and Nasal Antiseptic PI containing formulation or Betadine solution for 5 min. [PI solution has similar antistaphylococcal efficacy using ex-vivo porcine nasal vs. vaginal mucosa.] Tissues were washed with saline or saline containing 2% porcine mucin for 2 min and repeated after 15 min (to mimic mucocilliary clearance). Tissue was then infected with methicillin sensitive SA (MN8) 1 x 106 CFU/tissue (5mm). Tissue was incubated at 37oC for 1h; treated to neutralize PI, homogenized, and plated.
Results:
Betadine and 3M Skin and Nasal Antiseptic completely killed SA (>5 log reduction in CFU/tissue) without washes. Betadine solution had minimal effects on SA following saline washes; whereas, the 3M muco-adhesive PI nasal antiseptic reduced bacterial counts >3 log CFU/tissue (99.9%) by 1 h. Mucin 2% further reduced efficacy of Betadine, whereas, the 3M PI antiseptic was unaffected.
Treatment | Log reduction (CFU/tissue explant)* | |
Betadine | 3M Nasal Antiseptic | |
No wash | 5.33 (0) | 5.33 (0) |
Saline | 0.48 (0.13) | 3.19 (1.27)§ |
Mucin 2% | 0.10 (0.15) | 3.65 (1.28)§ |
*Average (+/- SEM) from untreated controls
§Statistically significantly different, p<0.05, n=3
Conclusions: Evidence suggests that washing reduces efficacy of PI containing antiseptics. The 3M Skin and Nasal Antiseptic performed significantly better than Betadine solution against SA using an ex-vivo tissue model designed to simulate mucocilliary clearance in the nares.